Rubella

1. Rubella haemagglutination inhibition tests     1.1 Individual reagents for ready-to-use rubella test kit - HIT     1.2 Rubella-Agglutest 2. Haemolysis-in-gel test for rubella 3. Rubella antigens 4. Control sera for Rubella tests

1. Rubella haemagglutination inhibition tests

The haemagglutination inhibition test detects antibody classes IgG and IgM. The test is based on the ability of the rubella antigen to agglutinate chick erythrocytes = haemagglutination. The agglutination is inhibited by specific rubella antibodies in the patient serum = haemagglutination inhibition (HI). Non-specific inhibition factors in the serum must be removed through an adsorption procedure.   (Download: Flyer "Rubella Haemagglutination Inhibition Tests", 915 kB)

1.1 Individual reagents for ready-to-use rubella test kit - HIT

Reagents are supplied for 25,50,75 or 100 assays.   (Download: Instructions for use "Rubella Haemagglutination Inhibition Test", 82 kB)

Name Description Unit Size Order No. Instructions for use Adsorption erythrocytes 10% Chick erythrocyte suspension ready-to-use 7,0 ml E-011   20,0 ml E-021   50,0 ml E-051             Adsorption buffer Heparin - manganese chloride buffer ready-to-use 7,0 ml P-030   50,0 ml P-031             Test erythrocytes 0.25 % Chick erythrocyte suspension ready-to-use 7,0 ml E-012   20,0 ml E-022   50,0 ml E-013             Test antigen Liquid, standardized 4-8 HA units, ready-to-use 5,0 ml RA-010   20,0 ml RA-012   50,0 ml RA-015             DGV albumin buffer Dextrose-gelatin-veronal buffer with albumin 50,0 ml P-020             Agglutest buffer ready-to-use 19,0 ml RT-001             Rubella antigen lyophilized for HIT and HiG for 1,0 ml RA-020 for 5,0 ml RA-520

1.2 RUBELLA-Agglutest

Name Description Unit Size Order No. Instructions for use RUBELLA-Agglutest Ready-to-use reagents for the haemagglutination inhibition test with lyophilized chick erythrocytes 20 assays RT-020 100 assays RT-200

2. Haemolysis-in-gel test for rubella

The haemolysis-in-gel test for rubella is particularly suitable for detecting low or borderline titres in the HIT test, which is required in accordance with German maternity guidelines. Through the use of chick erythrocytes in the test, non-specific reactions are rare in pathological sera (5%); they can be detected using the control plates. The test can also contribute to diagnostic clarification of a fresh infection: IgM antibodies, which arise on the 1st to 2nd day after the onset of illness, are detected by the HIT but not by the HiG. IgG antibodies are detected starting on the 2nd day in the HIT and on the 5th to 7th day in the HiG. A difference in titre between the two methods indicates an acute infection and requires further serological examinations.   (Download: Flyer "Haemolysis-in-gel test for rubella", 839 kB)

Name Description Unit Size Order No. Instructions for use Haemolysis-in-gel test for rubella Radial haemoolysis test 13 assays RH-100 Agar plate with rubella antigen-coated chick erythrocytes 6 assays RH-120           Control test package Agar plate with chick erythrocytes without antigen at least 13 assays RH-110 at least 6 assays RH-130

3. Rubella antigens

Name Description Unit Size Order No. Instructions for use Rubella antigen Lyophilized, for HIT with at least 256 HA units for 1,0 ml RA-020 for 5,0 ml RA-520           Rubella antigen 4-8 HA units, lyophilized, for HIT for 4,8 ml RT-003           Test antigen liquid, standardized, 4 – 8 HA-units for HIT 5,0 ml RA-010   20,0 ml RA-012   50,0 ml RA-015             Rubella antigen purification as agreed with customer Bulk

4. Control sera for Rubella tests

Serum of human origin is used to produce control sera. Errors and cross reactions are eliminated through strict quality control.

Name Description Unit Size Order No. Instructions for use Rubella-IgG-Kontrollseren 3 sera: high positive, low positive, negative; for HIT, HiG, EIA 3 x 100 µl RS-030   3 x 1000 µl RS-130