Diabetes

1. ELISA 2. Antigen, antibody

1. ELISA

ELIMMUN-Diabetes type I is a standardized, easy-to-run ELISA for the combined and simultaneous detection of autoantibodies to GAD65 and IA2 in a serum sample. The wells of the microtitre plates are coated with a recombinant fusion antigen consisting of tyrosine phosphatase and glutamate decarboxylase (IA2c:605-977 / GAD65:1-585). The test is based on the binding of specific IgG antibodies to the immobilized IA2-GAD fusion antigen. Binding is detected through a peroxidase-labelled anti-human IgG conjugate.   (Download: Flyer "Diabetes type I", 710 kB)

Name Description Unit Size Order No. Instructions for use ELIMMUN-Diabetes type 1 Detection of antibodies against IA2c (605-979) - GAD65 (1-585) 96 assays ED-100

2. Antigen, antibody

Name Description Unit Size Order No. Instructions for use GAD/IA2 fusion antigen Specificity: IA2c (605–979)/GAD65 (1–585) Purified antigen for ELISA, RIA, blot, immunoprecipitation 10 µg DI-330             Anti-GAD monoclonal, murine Specificity: GAD65 IgG1-Mab, purified for blot, ELISA 25 µg DI-300             Anti-GAD monoclonal, human Specificity: GAD65 (MICA 7) (483 – 585) IgG1-Mab, purified for ELISA, immunoprecipitation, immunochemistry 10 µg DI-207             Anti-GAD monoclonal human Specificity: GAD65 (MICA 8) IgG1-Mab, purified for ELISA, immunoprecipitation, immunochemistry 10 µg DI-208             Anti-GAD monoclonal human Specificity: GAD65 (MICA 10) (242 – 365) IgG1-Mab, purified for immunoprecipitation, immunochemistry, for ELISA 10 µg DI-210             Anti-GAD rabbit immunoserum Specificity: GAD65-Peptide, (325 – 340) for Blot, ELISA 50 µl DI-020             Anti-IA2 rabbit immunoserum Specificity: IA2-Peptide (959 – 977) for Blot, ELISA 50 µl DI-030